Gliadin component composition of Siberian varieties of spring soft wheat referred to various grain quality groups

The purpose of the study was to determine genetic struc- ture in terms of gliadin loci of spring wheat varieties of Siberi- an selection belonging to three groups of grain quality-strong, valuable and fillers. The problems were identifying alleles of gliadin loci by electrophoretic spectra; the analysis of the frequency of their occurrence in loci, in certain varieties and quality groups. The material included 23 varieties of wheat, created in 8 regions of Siberia. The electrophoresis of gliadins was carried out in polyacrylamide gel in aluminum-lactate buffer pH 3.1. For the identification of alleles, the reference variety Bezostaya 1 was used. It was established that all in- vestigated varieties had unique spectra, and, in addition to three, they were homogeneous in gliadins. Each locus was represented by 2-9 alleles, to analyze the relationship withgrain quality; alleles present in more than 9-20 genotypes were used: Gli-A1f, Gli-B1e, Gli-B1b, Gli-D1a, Gli-A2q, Gli- D2a...