Root formation of female plants of cloudberry (Rubus chamaemorus L.) in vitro

The paper presents the results of studies on clonal micropropagation of female plants of cloudberry (Rubus chamaemorus L.) at the stage of rooting microshoots in vitro. R. chamaemorus is an economically valuable forest berry plant in food and medicinal terms. Cloudberry cultivation in depleted peat deposits is promising. To obtain a large amount of planting material for plantation cultivation of forest berry plants, it is advisable to use the method of clonal micropropagation. It is necessary to improve the technology for growing R. chamaemorus in in vitro culture for forms of Northern Russian origin. The objects of study are plants of R. chamaemorus of the Arkhangelsk, Vologda, Karelian and Khanty-Mansiysk forms. At the stage of rooting of microshoots in vitro, the largest values of the number (3.7-6.0 pcs.) and total length (14.2-25.9 cm) of roots in female plants of R. chamaemorus were noted on the MS nutrient medium compared to options for diluting the mineral composition in 2 and 4 times. An increase in the concentration of IAA auxin in the nutrient medium from 0.5 to 1.0 mg/l contributed to a 1.4-1.8 times increase in the number of roots of female R. chamaemorus plants in culture in vitro, as well as an increase in the Karelian form (1. 3 times) and a decrease in the Arkhangelsk and Khanty-Mansi forms (1.2-1.4 times) in the total length of roots.