Correction of metabolic disorders of the articular cartilage and morphological evaluation of intra-ozone therapy in experimental post-traumatic arthritis

Introduction. Currently, increased medical and social significance of the problem of traumatic injuries of the knee joint. High prevalence, social losses due to temporary disability at a substantial cost of treatment and the risk of subsequent development of post-traumatic arthrosis, arthritis due to lack of pathogenetic approach to the conservative treatment of post-traumatic disorders in the joints make it extremely urgent the development of modern methods of diagnosis and treatment of knee joint injuries and prevent further progression of posttraumatic gonarthrosis. Materials and Methods. In the experiment on 132 white non-linear rats studied the content of hydroxyproline fractions in serum and morphological investigation of the articular cartilage, synovial membrane and subchondral bone layer during treatment with nimesulide, intra-articular ozone therapy, and combinations therapy. Results. The combined use of nimesulide and intra-ozone therapy in traumatic injury of the knee to a greater extent reduces alterations of collagen components of the extracellular matrix of cartilage and correction of synthetic processes in the metabolism of collagen, which prevents the processes of excessive fibrillogenesis and sclerosis of tissues damaged joint. Histological examination of the cartilage is affected areas and synovium were not significantly different from the cartilage of intact animals. The zoning of the structure of cartilage lesion is clearly discernible. Fibrillation of the surface and the intermediate zone was observed. In the study of subchondral bone degradation and bone sclerosis beams were found. Discussion and Conclusions. It was found that the combinations therapy of nimesulide and intra-ozone therapy in post-traumatic arthritis in the experiment prevents of destructive and degenerative changes in the joint, reducing biodegradation of the collagen fibers and blocking excessive proliferation of connective tissue and so promotes regeneration of the defect.