Determination of specificity of proteinase from Trichoderma viride by mass spectrometry

The possibility of using mass spectrometry to determine specific sites of hydrolysis of peptide and protein sequence was demonstrated. The types of digested bonds, scheme of hydrolysis, kinetic parameters for most easily hydrolyzable bonds were determined. The effect of pH on the initial rates of SPN hydrolysis was appreciated. The agreement of data from mass spectrometry and HPLC was demonstrated.