Mathematical simulation of cyclic regimes of temperatures regulation for polymerase chain reaction (PCR) for molecular colonies method (MCM) by microchip

For PCR realization it is necessary to ensured cyclic temperature change in correspondence with particular-linear law from 55 to 95 ºC within the predetermine time. This paper deals with estimation of heat-transfer dynamic in the object (reaction camera) with size 0.7-1.0 mm based on the calculated mathematic model. Temperature regulation is carried out by heaters, which are placed on the camera walls. Calculation results allow to estimate heat-transfer values for different schemes of temperature regulation. Basing on this information it is possible to minimize cycle-time and whole time of polymerase chain reaction, including molecular colony method.