Immobilized metal ion affinity chromatography (IMAC). Principle and applications
Автор: Keltsieva O.A., Gladilovich V.D., Podolskaya E.P.
Журнал: Научное приборостроение @nauchnoe-priborostroenie
Рубрика: Хроматография, ПЦР-, ДНК-анализ
Статья в выпуске: 1 т.23, 2013 года.
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This article reviews the basic principle of operation and the possibility of using immobilized metal affinity chromatography which include cleaning of histidine-tagged proteins, metal-binding proteins and antibodies. The advantages and disadvantages of this method and various sorbents are described.
Immobilized metal affinity chromatography (imac), sorbents, protein purification, phosphoproteomics
Короткий адрес: https://sciup.org/14264847
IDR: 14264847
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