Methods of sanitary surveillance of livestock production. VI. Immune-enzyme analysis of chloramphenicol

For monitoring of chloramphenicol residues, the conditions were optimized of an indirect immune-enzyme assay on the basis of polyclonal rabbit antibodies to chloramphenicol succinate conjugated with bovine serum albumin. Chloramphenicol succinate conjugated with a heterologous protein carrier, such as egg albumin, rabbit serum albumin and gelatin, was used as an immobilized antigen. A sensitivity of the assay makes 0.1 ng/ml. The possibility to use a developed immune-enzyme test-system in control of chloramphenicol residues in milk, meat and eggs is discussed.