Methods of statistical analysis of fluctuating asymmetry

Methodical problems concerning the practical use of fluctuating asymmetry level of bio-objects are considered. The questions connected with the variety of value asymmetry calculation methods and the use of asymmetry indicators efficiency and integrated indexes are discussed in detail. Discrepancy of research results when using several estimates of asymmetry is connected with their statistical properties and peculiarity of their normal variability which define sensitivity and operability of indicators. Concrete examples illustrating the negative influence of arithmetic transformations on the revealing properties of indicators are given: disturbance of normal distribution and the need of using rough nonparametric criteria, the increase of the importance of rare casual deviations, the introduction of additional variability components into an asymmetry level. Problems which arise in calculating asymmetry integrated indexes when signs unite with different levels of statistical parameters are separately considered. It is recommended to use the indicator of fluctuating asymmetry based on normalized deviation.