Modification of the method for determination of bactericidal activity of blood in agricultural animals

The authors modified the O.V. Bukharin and V.L. Sozikin method for the determination of bactericidal activity of blood in agricultural animals. The conditions for carrying out of method were optimized for the purpose of the most entire estimation of resistance degree in animals. It was shown that for estimation of blood bactericidal activity the following conditions should be keep: 90 min. before and 90 min. after addition of nutrient medium; a volume of blood plasma obtained from cattle, pigs, small cattle and horses must be amounted, respectively, 20, 50, 50 and 100 ц1; the blood plasma of pigs before testing must be centrifuged for fibrin pellet precipitation and avoidance of ge-latinization of reaction mixture; the volume of suspension of test-culture (concentration 1 milliard/ml) for all animal species must be 300 ц1, nutrient broth - 2000 ц1.