Molecular genetic identification of populations of Pinus sylvestrys L. in the east of the Russian plain based on polymorphism ISSR-markers

A molecular genetic analysis and identification of the four populations of Pinus sylvestris L., located in the Komi Republic and Kirov region, using ISSR-method of DNA polymorphism analysis. In four studied populations P. sylvestris identified 117 ISSR-markers; a high percentage of polymorphic loci (Р95 = 0.949). One ISSR-primer initiated synthesis in P. sylvestris on average 16.7 ISSR-markers. In the studied populations of P. sylvestris identified 16 rare ISSR-markers. Revealed the identity of the monomorphic species and polymorphic ISSR-markers, and combinations there of for the molecular genetic identification of the populations studied. Compiled by molecular genetic formula and barcodes four studied populations of P. sylvestris. The data obtained can be used to identify populations and wood of P. sylvestris in the studied region.