Analysis of tranagenic cell lines of bread wheat using IRAP PCR

By IRAP PCR with primers to LTR of SIRE 1 retrotransposon, at the three transformed lines showed the emergence of new, relatively high (more than 1000 bp) amplicons, which indicating of the TE transposition. As new DNA fragments were detected in three of seven studied lines and were absent in the control callus, it may indicate that the induction of transposition SIRE 1 may be due to genomic stress, caused by inserting foreign DNA (plasmid pAHC25) or stress associated with the transformation process (mechanical wounding, cultivation on selective media).