Molecular-genetic monitoring of salmon fishes associated microflora: diversity and physiological status

The urgency of introduction the molecular-genetic monitoring of associated fishes microflorae provides development of diseases molecular diagnostics and is caused first of all by threat of wide circulation of bacterial, virus and fungoid infections in conditions of the artificial contents. Development and introduction of the effective selective detection methodology on alive and viable bacterial cells on the basis of directed inhibition of extracellular DNA will allow to reveal quickly and correctly the activator of fishes infectious diseases and to monitor efficiency of carrying out the sanitary-and-epidemiologic actions. The method of DNA selective inactivation the death cells of organism-owner and bacteria with use of monoasid ethidiumbromid (МEB) is offered and approved on salmon fishes from aquarian expositions of Baikal museum ISC SB RAS. Conditions of DNA allocation are picked up, in group-and kind-specific PCR sets of target amplicons are received, series of experiments with laboratory culture of E. coli strain are lead. It is established, that at low concentration anavidin is an effective lytic reagent and on its basis the creation of a new set for allocation of total DNA is possible. The offered methodology will be demanded by development of modern high technologies of molecular-genetic monitoring of aquatic ecosystems.