Molecular participants of receptor pathway of apoptosis regulation in tumor and normal lympfocytes in inhibition of heat shock protein 90 in vitro

The study of programmed death of lymphocytes from healthy donors and tumor-cell line Jurkat in culture conditions with a selective inhibitor of Hsp90 (Heat shock protein - Hsp) 17-AAG was investigated. Assessment of apoptosis was performed by fluorescent microscopy using FITC-labeled annexin V and propidium iodide. Caspase-8 was determined by spectrophotometer assay. It has been established that inhibition of Hsp9 leads to activation of caspase-8, increasing the number of TNFR1-positive cells, which facilitates the launch of the programmed death of tumor cell line Jurkat.