Monitoring of virus symptoms in winter wheat variety sample from the collection of All Russian Institute of Plant Industry named after N. I. Vavilov

Selection of variety samples and sowing of winter wheat collection of funds N. I. Vavilov All-Union Research Institute of Plant Industry was conducted with the objectives of the definition: the productivity and quality of grain, phytosanitary characteristics (damage by pests (vectors), the prevalence of symptoms of viral, bacterial, fungal diseases), and promising accessions in the selection process. Identification of viruses, potentially affecting the wheat plants including be-longing to the genera Tritimovirus, Furovirus performed by visual agroecological monitoring and reverse transcription-polymerase chain reaction (RT-PCR). Of wheat plants with symptoms in the total RNA was isolated using the method described Rott and Jelkman (1990), which was used for RT-PCR as a template. Were selected for detection by using information on the field genebank primer coat proteins SBWMV, WSMV and synthesized by «Synthol» Moscow. As a result, a group of visually identified accessions with no signs of defeat viral diseases: Choc, Rafale (France); Leopold (Austria); Susquehanna, Norkan (USA), Cossack, Volga-Don (Volgograd); KNIISKH 756, Bystrica, villager, Krasnodar Scientific Research Institute of Agriculture 8 ( Krasnodar); Mironovskaya 34 Vasilina (Ukraine), Orenburg 14, Besouro 2 (Orenburg) Severodonskaya 14 (Rostov) and had a score of 0 on the proposed grade (tillering) - being the prime candidates for selection. In the steppe zone of the Southern Urals, the first time RT-PCR positive identification of pathogens - virus wheat streak mosaic (WSMV) and soil-wheat mosaic virus (SBWMV).