Morphological evaluation of invaginated anastomosis in colon surgery with angiostimulating technology in the experiment

The objective of the paper is to provide experimental and morphological grounds of angiostimulation in invaginated anastomosis formation in colon surgery. Materials and Methods. The study enrolled 53 mongrel dogs. The authors estimated the results of angiostimulation in formation of traditional or invaginated anastomoses after preliminary modeling of acute colonic obstruction. Results. Experimental studies showed that on the first day after left-sided obstructive colonic obstruction modeling and the subsequent formation of traditional or invaginated anastomoses in sigmoid colon almost single-type morphological response was observed in case of pharmagene use. However, during the blood channel injection it was found out that the functioning vessels were still absent without angiostimulation on the 7th day of regenerative process in the site of a mechanical damage. Whereas after pharmagene usage the vessels started apperaring on the 1st-3rd days, and by the 7th day the forming vascular tree was clearly visible. Morphometric cytogram analysis showed that in the early stages of monitoring the use of pharmagene stimulated plasmocytic, basophilic and fibroblastic reactions. All this, in turn, resulted in early neoangiogenesis, and ultimately to an earlier repair of the damaged colon interstitium. Conclusion. Pharmagene use may be well implemented under clinical conditions.