Morphological study of components of cellular and humoral immune response in rat spleen with experimental hypercalcemia

This research is devoted to a complex morphological assessment of components of cellular and humoral immunity rat spleen in response to the increase in calcium levels in the peripheral blood. The purpose of research - the study of the morphological characteristics of CD4-, CD8-, CD20-positive spleen cells of laboratory rats with experimental hypercalcemia on the background of long-term use of calcium from drinking water connection. It is proved that the experimental hypercalcemia accompanied by a redistribution of components of cellular and humoral immune response of the spleen: an increase in CD4+ cell of white pulp, CD8+ cells of red pulp by a decline in the number of CD20+ cells in all morpho-functional areas, except for the germinal centers. The revealed morphological changes in the spleen complex hypercalcemia indicates its selective nature of the impact on different parts of the immune response. There is increased activity of T-helper and T-cytotoxic lymphocytes with a compensatory decrease in indicators B-lymphocyte level that reflects a manifestation of adaptation and autoregulation of the immune response.