Multiherbal formulations of five plants ameliorate the effects on liver glycogen, serum protein, and lipid profile in alloxan-induced diabetic albino rats

Автор: Singh Radha, Singh Kusum, Ahirwar Vinita

Журнал: Журнал стресс-физиологии и биохимии @jspb

Статья в выпуске: 1 т.21, 2025 года.

Бесплатный доступ

Background: Diabetes mellitus is a non-communicable disease that is also referred to as a lifestyle disorder that requires modifications in diet, exercise, and behavior along with medication. As per the Indian traditional system of medicine, many medicinal plants have been used to manage various health disorders, including diabetes mellitus . Therapeutic plants are an important source of medicine, and various active compounds were isolated from the plants. The present research work was designed to evaluate the impact of multiherbal formulations on blood sugar and body weight in albino rats. Three different formulations were prepared from five plant extracts ( Allium sativum, Azadirachta indica , Phyllanthus emblica, Tamarindus indica, and Zingiber officinale ). After daily administration of multiherbal formulations at 300 mg/kg b.wt. and the standard drug (Glibenclamide) at a dose of 5 mg/kg b.wt. for 15, 30, and 45 days, respectively. Blood samples were collected from each rat and analyzed according to standard techniques (parameters), and biochemical parameters, viz., glycogen, protein, and lipid profile (serum cholesterol, triglyceride, HDL, LDL, and VLDL) of rats, were measured.

Еще

Antidiabetic properties, medicinal plants, formulations, diabetic rats

Короткий адрес: https://sciup.org/143183786

IDR: 143183786

Список литературы Multiherbal formulations of five plants ameliorate the effects on liver glycogen, serum protein, and lipid profile in alloxan-induced diabetic albino rats

  • Ahmed R.S., Patel U.D., Bhadarka D.H., Patel H.B. and Modi C.M. (2018). Modulation of antioxidant defense system by polyherbal extract mixture which ameliorated the pathophysiological alterations in streptozotocin-induced diabetic rats. Annals of Phytomedicine, 7(2), 102-113.
  • Al-Ahdab M.A. (2015). Anti-Hyperglycemic Effect of Tamarindus indica Extract in Streptozotocin-Induced Diabetes in Male Rats. World Applied Sciences Journal, 33(12), 1940-1948.
  • Alhamhoom Y., Ahmed S.S., Kumar R.M., Salahuddin M.D., Bharathi D.R., Ahmed M.M., Farhana S.A. and Rahamathulla M. (2023). Synergistic antihyperglycemic and antihyperlipidemic effect of polyherbal and allopolyherbal formulation. Pharmaceuticals, 16, 1368.
  • Amrutha Raj P., Jayachandran T.P. and Soniraj V.S. (2022). Evaluation of antidiabetic potential of polyherbal formulation (diaronil) in alloxan-induced rat model. World Journal of Pharmaceutical Research, 11(3), 1829-1845.
  • Anfenan M.L.K. (2014). Evaluation of nutritional and antidiabetic activity of different forms of ginger in rats. Middle-East Journal of Scientific Research, 21(1), 56-62.
  • Anwer T., Alkarbi Z.A., Najmi A.H., Alshahrani S., Siddiqui R., Khan G. and Alam M.F. (2019). Modulatory effect of zingerone against STZ nicotinamide induced type-2 diabetes mellitus in rats. Archives of Physiology and Biochemistry, 2019, 1-7.
  • Babu P.S. and Prince P.S.M. (2004). Antihyperglycaemic and antioxidant effect of hyponidd, an ayurvedic herbomineral formulation in streptozotocin-induced diabetic rats. Journal of pharmacy and pharmacology, 56, 1435-1442.
  • Baligar N.S., Aladakatti R.H., Ahmed M. and Hiremath M.B. (2014). Evaluation of acute toxicity of neem active constituent, nimbolide, and its hepatoprotective activity against acute dose of carbon tetrachloride treated albino rats. International Journal of Pharmaceutical Sciences and Research, 5(8), 3455-3466.
  • Bhat M., Kothiwale S.K., Tirmale A.R., Bhargava S.Y. and Joshi, B.N. (2011). Antidiabetic Properties of Azardiracta indica and Bougainvillea spectabilis in in-vivo studies in murine diabetes model. Evidence-Based Complementary and Alternative Medicine, 2011, 1-9.
  • Bisht S. and Sisodia S.S. (2010). Anti-Hyperglycemic and Antidyslipidemic Potential of Azadirachta indica Leaf Extract in STZ- Induced Diabetes Mellitus. Journal of Pharmaceutical Sciences and Research, 2(10), 622-627.
  • Burstein M., Scholnic H.R., Morfin R.J. (1970). Lipid Research. Chakraborty D., Mukherjee A., Sikdar S., Paul A., Ghosh S. and Khuda-Bukhsh A.R. (2012). [6]-Gingerol isolated from ginger attenuates sodium arsenite-induced oxidative stress and plays a corrective role in improving insulin signalling in mice. Toxicology Letters, 210, 34-43.
  • Chandra T. and Sadique J. (1987). A new recipe for liver injury. Ancient science of life, 7(2), 99.
  • Choudhari V.P., Gore K.P. and Pawar A.T. (2017). Antidiabetic, antihyperlipidemic activities and herb-drug interaction of a polyherbal formulation in streptozotocin-induced diabetic rats. Journal of Ayurveda and Integrative Medicine, 8, 218-225.
  • Dhanavathy G. (2015). Immunohistochemistry, histopathology, and biomarker studies of swertiamarin, a secoiridoid glycoside, prevents and protects streptozotocin-induced p-cell damage in Wistar rat pancreas. Journal of Endocrinological Investigation, 38(6), 669-684.
  • Ekambaram P., Namitha T., Bhuvaneswari S., Aruljothi S., Vasanth D. and Kumar M.S. (2010). Therapeutic efficacy of Tamarindus indica (L) to protect against fluoride-induced oxidative stress in the liver of female rats. Research report Fluoride, 43(2), 134140.
  • Friedewald W.T., Levy R.I., Fredrickson D.S. (1972). Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without the use of the preparative ultracentrifuge. Clinical Chemistry, 18, 499-502.
  • Grzanna R., Lindmark L., and Frondoza C. (2005). Ginger-An herbal medicinal product with broad antiinflammatory actions. Journal of Medicinal Food, 8(2), 125-132.
  • Gupta A.D., Dhara P.C., Dhundasi S.A. and Das K.K. (2009). Effect of garlic (Allium Sativum) on nickel П or chromium VI induced alterations of glucose homeostasis and hepatic antioxidant status under sub-chronic exposure conditions. Journal of Basic & Clinical Physiology & Pharmacology, 20(1), 1-14.
  • Gupta V. (2010). Hypolipidemic effect of garlic: on experimentally induced hyperlipidemia. Journal of Advance Researches in Biological Sciences, 4(1), 56.
  • Herbert K. (1984). Lipids, in chemical chemistry: Theory, Analysis, and Co-relation. Kalpan L.A. and Pesce A.J. (Eds.), C.V. Mosby: Toronto, pp. 1182-1230.
  • Iftikhar A., Aslam B., Muhammad F., Khaliq T., Faisal M.N., Rahman Z.U., Khan J.A. and Majeed W. (2019). Biochemical and histopathological investigations of antidiabetic potential of polyherbal formulation in alloxan-induced diabetic rats. Pakistan Journal of Agricultural Sciences, 55(3), 761-766.
  • Jain R., Pandey R., Mahant R.N. and Rathore D.S. (2015). A review on medicinal importance of Emblica officinalis. International Journal of Pharmaceutical Sciences and Research, 6(1), 7284.
  • Jeyaraj S., Shivaji G., Jeyaraj S.D. and Vengatesan A. (2006). Effect of combined supplementation of fish oil with garlic pearls on the serum lipid profile in hypercholesterolemic subjects. Indian Heart Journal, 57(4), 327-331.
  • Kaliaperumal K., Bhat B.A., Subramanian K., Ramakrishnan T., Chakravarthy E., Al-Keridis L.A., Ahmad I., Alabdallah N.M., Mohd Saeed M., Karunakaran R. (2024). In-vivo anti-hyperglycemic effect of herbal extracts Tribulus terrestris (L) and Curcuma amada (R) on streptozotocin-induced diabetic rats and its associated histopathological studies. Heliyon, 10(1), 1-11.
  • Kazeem M.I., Akanji M.A., Yakubu M.T. and Ashafa A.O.T. (2013). Protective effect of free and bound polyphenol extracts from ginger (Zingiber officinale Roscoe) on the hepatic antioxidant and some carbohydrate metabolizing enzymes of streptozotocin-induced diabetic rats. Evidence-Based Complementary and Alternative Medicine, 2013, 1-7.
  • Khattab H.A.H., Al-Amoudi N.S. and Al-Faleh A.A. (2013). Effect of Ginger, Curcumin and Their Mixture on Blood Glucose and Lipids in Diabetic Rats. Life Science Journal, 10(4), 428-442.
  • Koyaguru N., Kumar V.H., Jamadar M.G., Huligol S.V., Nayak N., Yendigeri, S.M. and Shamsuddin M. (2013). Antidiabetic and hepatoprotective activities of Tamarindus indica fruit pulp in alloxan-induced diabetic rats. International Journal of Pharmacology and Clinical Sciences, 2(2), 33-40.
  • Lebda M.A., Taha N.M., Korshom M.A., Mandour A.E.W.A., and Goda R.I. (2013). Ginger (Zingiber officinale) potentiates paracetamol-induced chronic hepatotoxicity in Rats. Journal of Medicinal Plants Research, 7(42), 3164-3170.
  • Lowry O.H.N., Rusenbough J., Farr A.L. and Randall R.J. (1951). Protein measurement with folin phenol regent. J. Biol. Chem, 193(1), 265-275.
  • Lupascu F.G., Giusca S.E., Caruntu I.D., Alina A., Lupusoru C.E. and Profire L. (2019). The safety profile of new antidiabetic xanthine derivatives and their chitosan-based formulations. European Journal of Pharmaceutical Sciences, 127, 71-78.
  • Maiti R., Das U.K. and Ghosh D. (2005). Attenuation of hyperglycemia and hyperlipidemia in streptozotocin-induced diabetic rats by aqueous extract of seed of Tamarindus Indica. Biol. Pharm. Bull, 28(7), 11721176.
  • Majeed W., Khaliq T., Aslam B., and Khan J.A. (2018). Polyherbal formulation prevents hyperglycemia by modulating the biochemical parameters and upregulating the insulin signaling cascade in alloxan-induced hyperglycemic rats. Pakistan Veterinary Journal, 38(2), 121-126.
  • Manorama P., Aruna V., Geetha D and Angajala G. (2022). HPLC profiling and antihyperglycemic evaluation of PHE (polyherbal extract) of selected Indian medicinal herbs in alloxan diabetic rats. Rasayan J. Chem. 15(4), 2576-2583.
  • McGowan M.W., Artiss J.D., Strandbergh D.R. and Zak B. (1983). A peroxidase-coupled method for the colorimetric determination of serum triglycerids. Clinical Chemistry, 29(3), 538-542.
  • Meher B. and Dash D.K. (2013). Antihyperglycemic and Hypolipidemic effects of Tamarindus indica L.: A Potential agent for treatment of metabolic syndrome. International Journal of Pharmaceutical Innovations, 3(6), 30-50.
  • Mishra B., Hegde S., Harsha M. R., Ramana V., and Chaithra C. S. 2016. Therapeutic uses and action of Neem on skin diseases vs. InnoVision Neem capsule/Tablet. International Journal of Innovative Research Multidisciplinary Field. 2(8), 124-128.
  • Momoh J.O., Manuwa A.A., Oshin T.T. (2022). Phytochemical screening, gas chromatography: mass spectrometry and antidiabetic properties of aqueous extract of ginger (Zingiber officinale) in alloxan-induced diabetic Wistar rats. Journal of Pharmacognosy and Phytochemistry, 11(5), 11-19.
  • Muthukumaran P. and Begum V.H. (2020). Effect of Poorna Chandrodayam Chendooram (PCM - Metallic Drug) on Lipid Profile, Liver Function and Kidney Function Parameters of Rats. Asian Journal of Pharmaceutical Analysis, 10(1), 27-31.
  • Narasimhacharya A.V.R.L. and Vasant R.A. (2012). Amla is an antihyperglycemic and hepato-renal protective agent in fluoride-induced toxicity. Journal of Pharmacy Bioallied Sciences, 4(3), 250-254.
  • Padiya R. and Banerjee S.K. (2013). Garlic as an anti-diabetic agent: recent progress and patent reviews. Recent Patents on Food, Nutrition, and Agriculture, 5(2), 105-127.
  • Reddy V.D., Padmavathi P., Paramahamsa M., and Varadacharyulu N.C. (2010). Amelioration of alcohol-induced oxidative stress by Emblica officinalis (Amla) in rats. Indian Journal of Biochemistry & Biophysics, 47, 20-25.
  • Resny A.R., Indulekha V.C. and Raj R.V.B. (2018). A critical Ayurvedic literary review of the plant amleeka (Tamarindus Indica L.). International Ayurvedic Medical Journal, 2(3), 1060-1067.
  • Roberts, W.L., et al. (2008). Reference information for the clinical laboratory in Teitz Fundamentals of Clinical Chemistry. Burtis C.A. et.al. (6th eds.), pp.861.
  • Saeid J.M., Mohamed A.B. and AL-Baddy M.A. (2010). Effect of Aqueous Extract of Ginger (Zingiber officinale) on Blood Biochemistry Parameters of Broiler. International Journal of Poultry Science, 9(10), 944-947.
  • Saravanan G., Ponmurugan P., Kumar G.P.S. and Rajarajan T. (2009). Antidiabetic properties of S-allyl cysteine, a garlic component on streptozotocin-induced diabetes in rats. Journal of Applied Biomedicine, 7, 151-159.
  • Saravanan G., Ponmurugana P., Kumar G.P.S. and Rajarajan T. (2009). Modulatory effect of S-allyl cysteine on glucose metabolism in streptozotocin-induced diabetic rats. Journal of functional foods, 1, 336-340.
  • Sarfraz M., Ullah H., Iqbal R., Shaheen G., Albalawi, M.A., Alatawi F.S. et al., (2024). Antidiabetic effect of black pepper, turmeric, and ajwa date pulp, seed, and their mixtures as antioxidants in alloxan diabetic rats. Journal of Animal and Feed Sciences, 33(1), 28-46.
  • Seifter S., Dayton S., Novic B. and Muntwyier E. (1950). The estimation of glycogen with anthrone reagent. Arch. Biochem. 25, 191.
  • Seyidoglu N., Karakci D., and Bakir B. (2023). Role of ginger in ameliorating streptozotocin-induced diabetic rats. International Journal of Herbal Medicine, 11(1), 61-66.
  • Shadli S., Alam M., Haque A., Rokeya B. and Ali L. (2014). Antihyperglycemic Effect of Zingiber officinale Roscoe Bark in Streptozotocin-induced Type 2 Diabetic Model Rats. International Journal of Pharmacy and Pharmaceutical Sciences, 6(1), 711716.
  • Shailey S. and Basir S.F. (2011). Long-term effect of Azadirachta indica on hyperglycemia, associated hyperlipidemia, and tissue glycogen content in alloxan-induced diabetic rats. Journal of Pharmacy Research, 4(3), 810-812.
  • Singh B., Ahamad A. and Pal V. (2015). Evaluation of antibacterial activity and phytochemical screening of Azadirachta indica leaves extracts against Staphylococcus aureus. UK Journal of Pharmaceutical and Biosciences, 3(4), 43-47.
  • Singh V. K. and Singh D. K. (2008). Pharmacological effects of garlic (Allium sativum L.). ARBS Annual Review of Biomedical Sciences, 10, 6-26.
  • Sri K.V.S., Kumari D.J. and Sivannarayana G. (2013). Effect of Amla, an approach towards the control of Diabetes mellitus. International Journal of Current Microbiology and Applied Sciences, 2(9), 103-108.
  • Suman M., Shivalinge G.K.P., Paul U. and Priyanka S. (2016). Evaluation of Antidiabetic and Antihyperlipidemic Activity of Newly Formulated Polyherbal Antidiabetic Tablets in Streptozocin-Induced Diabetes Mellitus in Rats. Asian Journal Pharmaceutical Clinical Research, 9(1), 201-207.
  • Yerima M., Anuka J.A., Salawu A.O. and Abdu-Aquye I. (2014). Antihyperglycaemic activity of the stem-bark extract of Tamarindus indica L. on experimentally induced hyperglycaemic and normoglycaemic Wistar rats. Pakistan Journal of Biological Sciences, 17(3), 414-418.
  • Zlatkis A., Zak B. and Boyle A.J. (1953). A new method for the direct determination of serum cholesterol. J.Lab.Clin.Med, 41, 486.
Еще
Статья научная