Multipotent mesenchymal stromal cells isolated from subcutaneous fat of mammals for the study of Sarcoptes scabiei/mange in vitro

Sarcoptes scabiei / mange is a small, roundish, pale gray mite that lives in the epidermis of mammalian skin and causes scabies. Despite the fact that the mite biology is well studied and its interaction with the host is intensively investigated, the data analysis demonstrates the absence of a cell culture on which the Sarcoptes scabiei / mange could multiply in vitro. This hinders the development of modern effective methods of diagnosis of this disease, the study of the immune response after infection in order to create vaccines and evaluate the effectiveness of the use of drugs for the clinical treatment of mite-borne disease. In this regard, the search for a cellular system that would maintain the viability of Sarcoptes scabiei / mange in vitro is particularly relevant. We describe cellular systems represented by multipotent mesenchymal stromal cells (MMSCs) that can be used for these purposes. MMSCs were isolated from subcutaneous adipose tissue (SAT) of cattle and humans...