A new approach for improvement of the methodology to identify a type of interaction of lactic acid bacteria in consortiums of different stages of cultivation

Existing approaches for evaluation of types of interactions between individual monocultures in consortiums allow obtaining only qualitative results (synergistic, antagonistic, additive interaction) as a whole, without regarding to changes in the cultivation process. Therefore, the development of a new approach for the quantitative determination of this indicator as a continuous function defined during the all period of cultivation is in need. In the course of the research a two-component consortium of lactic acid microorganisms cultivated on different mediums according to directed fermentation process in vegetable products was chosen to analyze types of interaction. As a result, the an original approach that was based on comparison of grow speed of biomass of microorganisms with calculated additive curve determined by results of dynamic analysis of titre of microorganism participating in consortium in monoculture during their cultivation has been elaborated. This approach is a convenient tool to identify complex regularity in changes of types of microorganism interaction in consortium represented by continuous function defined during all cultivation period.