To the role of calcium channel inhibitors in pharmacological correction of experimental nephrolithiasis

The aim of present investigation was to study niphedipine's effect on the experimental oxalate nephrolithiasis. Experiments were performed on 30 male rats, which were divided in 2 groups. 1% solution of ethylenglycole (EG) was given as a drink to rats of the control group during 6 weeks. Experimental group was given EG during the six weeks and from the 4th week niphedipine in dose of 10 mg/kg was prescribed. Every 3-4 days oxalate, phosphate and calcium concentrations, activity of urothelium injury's marker enzymes: lactate dehydrogenase (LDH), γglutamyl transferase (GGT), N-acetyl-β-D-glucose aminidase (NAG), and oxygen's free radicals activity were detected in daily urine's portion. Moreover, hystochemistry Van Koss method detected calcium deposits in the rats' kidneys. Treatment of experimental nephrolithiasis by niphedipine led to alleviation of pathology. It was indicated by the significant reduction of urine's oxalate concentration, pronounced decrease of marker enzymes activity: LDH, GGT, NAG, and reduction of oxidative stress. Finally, morphological studies showed very significant reduction of calcium's deposits' number and their sizes. The results of the present research revealed niphedipine's therapeutically effect on the experimental oxalate nephrolithiasis.