Adenylate cyclase activity in sheep platelets following external whole-body G-irradiation with different doses

The paper presents results of study of adenylate cyclase activity in the platelets after external whole-body g-irradiation of 32 tsigai rams (Ovis aries) with 2, 4 and 6 Gy, at the dose rate of 1 Gy/hour. The irradiation caused the development of acute radiation damage of a different degree of severity. To detect changes in the basal and prostoglandin E1 stimulated (PGE1-stimulated) adenylate cyclase activity thin-layer chromatography (TLC) was carried out during the first 30 days of the damage development. After external g-irradiation directly proportional effect of dose on changes in the basal adenylate cyclase activity in the platelets was registered on the 1 and the 7 days of the damage development, the changes in the PGE1-stimulated activity was registered on the 7 day. The study results allow making the suggestion that detected changes in the adenylate cyclase activity in the platelets of irradiated rams may be resulted from the modification of cytoplasmic cell membranes and impaired functioning of membrane-bound cAMP-sensitive signaling pathway. It may be also caused by new cells with qualitatively new properties released from the bone marrow megakaryocytes pool into the bloodstream.