Тhe assessment of functional status of Chlorella vulgaris suboblonga and Phaeodactylum tricornutum at acclimation to low temperature

The possibility of using fluorescein diacetate (FDA) (flow cytometry) and the coefficient of variable chlorophyll fluorescence (k) for a rapid assessment of the functional state of the algae Chlorella vulgaris suboblonga and Phaeodactylum tricornutum acclimated to low temperature were investigated. Sublethal temperature and light did not significantly impact the viability and integrity of the membranes of investigated cultures. This is evident by an increase in fluorescence emission intensity FDA by cultures growth. Changing the FDAfl, may be treated as a compensatory response of cells to the action of temperature. Coefficient of variable fluorescence had high values when the rate of cell division was maintained.