The assessment of morphofunctional changes in pigs’ placenta of during pregnancy complicated by isoimmunization

It is known that pathological course of pregnancy is connected with the disturbance in interdependent "mother-fetus" complex and leads to specific clinical consequences in molecular-cellular, tissue, organ, organism and population levels of its organization. In case of violation of placentation (placental barrier), the state of immune conflict occurs, characterized by antigen-antibody reaction and carried out through the placenta (in relation to the fetus), or through colostrum after birth (in relation to the newborn offspring). Therefore, immunological relations at the population level both the consequences of the action of the maternal organism's antigens on the fetus, and the effects of allogeneic action of the fetus on the mother's body should be considered. The material for the research was selected in the conditions of the collective farm SEC "Vostok" of Kursk district of Stavropol Region. The research was conducted at the Laboratory of Histology of Scientific Diagnostic and Therapeutic Veterinary Center of FSBEI HE ‘Stavropol State Agrarian University’. According to the results of the experiment, the placentas of 10 sows of large white breed were used as the material, which showed the signs of isoimmunization to the resulting offspring. For histological examination, immediately after the delivery, the pieces of placenta up to 0.5 cm thick were selected, which were fixed in a 10 % aqueous solution of neutral formalin. Fixed material after wiring through alcohols of increasing concentration, xylene, xylene-paraffin, was poured into paraffin. The obtained preparations were stained with conventional methods-hematoxylin and eosin. Describing morphofunctional state of the placenta, the structure, cellular composition, and state of blood vessels was made. The results obtained show the presence of changes: the reduction of cellular elements with loosening of stromal base and focal atrophy; post-inflammatory lymphoid-lymphocytic infiltrates; involution process; significant deposition of fibrinoid (fibrinoid masses).