Assessment of morphological changes in internal organs when studying the acute toxicity of the feed additive «Active Mix» VMG 500/600 in experiments on rats

It was necessary to study the safety of using these liquids in experiments on laboratory animals. Morphological assessment of the internal organs of white nonlinear laboratory rats when studying the acute toxicity of the high-calorie vitamin and mineral feed additive «Active Mix» VMG 500/600. The study was carried out on 70 non-linear white rats (females) weighing 206,2±17,8 g, which were divided into 7 groups (6 experimental and 1 control) of 10 animals each. Animals of the 1st (control) group were injected with physiological solution intragastrically through a tube; rats of the 2nd, 3rd and 4th groups were administered «Active Mix» VMG-500 in dosages of 30,000 mg/kg, 60,000 mg/kg and 120,000 mg/kg, respectively, and animals of the 5th, 6th, 7 group - «Active Mix» VMG-600 in similar dosages. Observation was carried out for 14 days. However, all the animals survived. On day 15, the rats were removed from the experiment. A histological examination of the heart, kidneys, and large intestine of animals in the experimental groups did not reveal significant morphological changes. In rats of the 2nd, 3rd and 4th groups, moderately pronounced disturbances of the microstructure of the epithelium of the gastric and small intestinal mucosa, liver and spleen parenchyma were noted. In animals of groups 5, 6, 7, no significant changes were observed. The results obtained indicate the safety of the feed additive and the possibility of its negative impact only with a very significant overdose of one of its parts «Active Mix» VMG-500.