Antitumor, toxicity and target gene expression evaluation of MIR-204-5P mimic application on melanoma B16-bearing mice

Objective. to evaluate anti-tumor, toxic effect of miR-204-5p mimic applicaton on melanoma B-16-bearing mice followed by miR-204-5p target gene expression estimation in melanoma tumor and distant organs. material and methods. c57Bl/6 melanoma B-16-bearing mice were used. the animals of the experimental group were intraperitoneally injected with a 5 nm miR-204-5p miRNa simulator (mimic) on the 8th, 10th, and 12th days after melanoma B-16 cell transplantation. Based on the results of bioinformatic analysis, miR-204-5p target genes BCL2 and SIRT1 expression levels were determined by quantitative real-time pcR. the toxic effect of miR-204-5p mimic was estimated by the evaluation of body weight, mass of the internal organs, and motor activity. results. on the 13-14th days of the experiment, the motor activity of animals in the control groups decreased significantly compared to the group of animals treated by miR-204-5p. target gene BCL2 showed increased expression in the lungs and kidneys and SIRT1 levels were increased in the lungs of miR-204-5p mimic treated animals (p˂0.05). tumor mass tended to decrease in the animals treated by miR-204-5p mimic. conclusion. modulation of the level of miR-204-5p microRNa led to changes in the expression of siRt1 and Bcl2 in the lungs of animals, and changes in the expression of Bcl2in the kidneys. miR-204-5p mimic application did not have toxic effect on animals treated. Further studies are necessary to clarify miR-204-5p implication in melanoma cell proliferation regulation as well as it’s biodistibution in the tumor tissue.