Phytotoxic activity reduction assessment of copper ions through the onion apical meristem

This paper investigates the protective properties of the aqueous extract of silkworm pupae (CABG) in terms of the toxic effect of copper ions on morphometrically parameters in Allium-test. Methods: It is established that the joint use of aqueous extract of silkworm pupae and copper sulphate at a concentration of 100 µm growth inhibition of roots is not happening and even stimulated by the application of aqueous extract of silkworm pupae at a concentration of 1 ml/10 ml. Stimulation of root growth is also observed with the combined use of 1000 µm copper sulfate and aqueous extract of silkworm pupae in a concentration of 10 ml/100 ml of the solution with respect to the use of copper sulfate only. A significant decrease in the parameter ‘length of sprouts' in 1.3-6.0 times with respect to the control was observed when using copper ions in concentrations of 100-1000 microns, while at a metal concentration of 10 microns no negative effect on the development of sprouts was established...