Estimation of transgenic soybean plants (Glycine max (L.) Merr.).) for resistance to phytopathogens

The aim of this work is to evaluate in vitro transgenic soybean plants developed using method of agro-bacterial transformation and containing gene AMP-1 in its genome (gene AMP-1 was provided by the FSBSI ARSIAB, Moscow), under control of the constitutive promoter 335 CAMV and NOS terminator, which is able to increase the limits of plant resistance to phytopathogens. As the transformation objects, there were used soybean cotyledonary nodes of cultivars Primorskaya 81 and Primorskaya 28. To prove integration and expression of AMR-1, we conducted RT-PCR analysis of cDNA samples allocated from plants of the regenerated line. Stable transformation of soybean in T1-T5 was confirmed. Immunological analysis proved that leaves from the transformant-plants were damaged by Septoria glycines Hemmi 25.7% less than standard cultivar, by Cercospora sojina Hara and Peronospora manshurica (Naum). Syd. - 20.0% less than standard. This proves that gene expression leads to the resistance increase of soybean transgenic plants to fungal pathogens Septoria glycines, Cercospora sojina and Peronospora manshurica.