Impact of microflora of environmental medium on ambulatory airway colonization in patients with cystic fibrosis

The aim of the study is to evaluate possible risks of respiratory tract microbial colonization in patients with cystic fibrosis. Materials and Methods. The authors conducted microbiological studies of 176 samples from family members and swabs from various objects taken in 17 families, in which patients with cystic fibrosis live. The research was conducted in Samara region. Each sample was analyzed for following culture media: 5 % blood agar, universal chromogenic culture medium, OFPBL-agar for elective isolation of B. sepacia complex bacteria, chocolate agar, and Saburo medium for fungi cultivation. Inoculations were incubated for 24-48 hours; the temperature was 37 and 28 °C. On OFPBL and Saburo media inoculations were left for incubation for 14 days. MALDI-Tof-mass spectrometry (Microflex, Bruker) was used to identify the isolated cultures. In general, 404 microbial strains were isolated. Among them there were many strains of non-fermentative gram-negative bacteria, which are of clinical importance in case of cystic fibrosis, namely Achromobacter spp...