The assessment of 1,2,3-triazolo-[5,1-b]-1,3,4-thiadiazine derivative influence on restoration of the fibrous skin component during regeneration caused by burn injury

In this study, the stimulating effect of the 1,2,3-triazolo-1,3,4-thiadiazine derivative on the restoration of the fibrous skin component was evaluated on the burn cutaneous wound model of white male rats. The experiment included morphological and mechanical investigations. The animals of the experienced groups were taken out of the experiment on the 14th and 21st days to assess the regenerative process. Experimental animals received a third A grade burn injury. Treatment was carried out by applying an ointment containing the test substance to the affected area. Morphological analysis data indicate the stimulating effect of 1,2,3-triazolo-1,3,4-thiadiazine on the skin regeneration, particularly, the fibrous component restoration. By the 14th day of the experiment, all samples have weak mechanical characteristics. However, on the 21st day, samples from the "triazole" group demonstrate higher strength properties, stretching by 28,893 ± 4,548 % at a maximum load of 0,804 ± 0,129 MPa. Significant difference from the intact value (31,434 ± 4,921 % extension at a load of 5,082 ± 0,060 MPa) is probably associated with the contribution to the mechanical properties not only of the fibers thickness, but also the ways of fibers stacking in bundles, arrangement and interaction with other structural components of the tissue.