Dandelion Taraxacum officinale L. as a promising tool for bio-diagnostics of the state of the urban environment

The need to expand the list of organisms used in environmental biodiagnostics arises from the variety of pollutants and types of man-made effects on the biosphere. The choice of available test organism should be based on easily detectable and differentiable sensitivity to factors, high reproducibility of seed material, alignment of the genetic line used by the gene pool and phenotype. The paper presents the results of the soil contamination of urban agglomeration study by the bioindication and biotesting method using a dandelion as a bioindicator plant and a test organism. Within the study, the generation of dandelion was obtained and investigated for applicability in biotesting. During the first stage of the study, soil samples were taken in the influence zone of VOAO Khimprom and near the 2nd Prodol’naya avenue of Volgograd. Germination of dandelion seeds showed noticeable differences in growth rates both in comparison with control and in comparison, of prototypes with each other. At the end of the first stage, numerous apomictic seed offspring were obtained toexcrete the aligned genetic lineage of test organisms. The expansion of the list of contaminated locations by counting the territories adjacent to the VMK Krasnyi Oktyabr’ showed the detected differentiated physiological reaction of test organisms grown in experimental soil samples. The results of the study confirm the postulate on soil contamination of urban locations with physiologically active pollutants and also characterize the used plant as a sensitive and easily reproducible test organism under artificial conditions. During the two-year experiment the possibility of selecting the genetic lineage Taraxacum officinale with predictable and monotonous properties was proved due to the tendency of this species to apomixis.