Oligonucleotide primers for detection of biphenyl 2,3-dioxygenase large subunite encoding genes of bacteria belonging to the order Actinomycetales

Biphenyl 2,3-dioxygenase (BDO) is a key enzyme involved in bacterial degradation of biphenyl/polychlorinated biphenyl (PCB). The structure of the active site determines the range of recyclable PCB congeners. We have designed the degenerate PCR-primers to target a region, encoding the large subunit of biphenyl dioxygenase. The oligonucleotides will be applied to screen to BDO large subunit encoding genes of bacteria belonging to the order Actinomycetales