Ontogenetic peculiarities of the formation of the rostos-sternal complex in alloxanic diabetes mellitus

The morphofunctional active point in the formation of the chest is the sternocostal complex. Changes in the shape and functional structure of the chest affect the functional state of the chest cavity organs. The lack of data on the morphofunctional properties of the sternocostal complex leads to serious shortcomings and errors in the prevention and treatment of injuries and deformities in certain areas.

MATERIALS AND METHODS

The study was carried out on 42 white laboratory rats weighing 150-200 g. For the study, the animals were divided into 2 groups. On the fifth day of gestation, the rats were induced experimental diabetes mellitus using the alloxan model. In the experimental group, a mixture of alloxan 150 mg / kg was injected intraperitoneally by a single intraperitoneal injection.

The material for the study was the breast components of 7–14–21–30–45 days of age in young rats born to mothers with experimental diabetes.

RESULTS AND DISCUSSION

In fact, the growth zone in all bones plays an important role in the formation and growth of bones, so the state of the structures of the sternocleidomastoid ridge has been studied. In morphological examination for histological examination of the thorax regions of the sternum complex of rats in the initial period of observation, the normative formation of ribs in the control animal during this period corresponds to the observation period (Fig. 1).

Staining method-hematoxylin-eosin. staining by the PIC method. Increased 200 times.

Magnification 200 times.

At a later date (45 days), chondrocytes have a spherical or round nucleus surrounded by cytoplasm. The growth zone is represented by proliferative cells, which are columnar and consist of 10-12 cells in each column. These chondrocytes differ from the adjacent columns by a clear set of fibrils oriented longitudinally along the layers of the main matrix material. In some chondrocytes of this zone, mitotic patterns were revealed. Specific cases of mitosis were revealed in rats of the main group [6]. Cartilage cracks are found between the columns of chondrocytes. In places, local dystrophic changes in chondrocytes are detected, the vascular wall thickens, fibrous structures decrease, and plasmorrhagic foci appear around the vessels (Fig. 2).

CONCLUSIONS

During the observation periods, the process of differentiation in the bone trabeculae slows down sharply, and therefore the range of the growth zone of the sternum expands in the distal and proximal directions. The data obtained indicate that during pregnancy in rats with maternal alloxan diabetes, the development of all structural structures of the growth zones of the sternocostal complex is delayed.