Determination of the antibacterial activity of flavonoids from callus Scutellaria galericulata L. for the development of a functional curd product

The antimicrobial effect of extracts from callus of the common skullcap with the prospect of using in the recipe of a functional curd product for therapeutic and preventive action is studied. It is known that plants of the genus skullcap contain phenolic compounds that cause antimicrobial action, the most common among them are flavonoids-baykalin, scutellarein, hydrolyzed to glucuronic acid and aglycones-baykalein and scutellarein, vogonin, etc. the Study of antimicrobial activity was carried out in accordance with the requirements of The state Pharmacopoeia XI edition. Antimicrobial activity was determined in relation to 15 test cultures by diffusion in agar. The method is based on the assessment of inhibition of growth of test cultures of microorganisms by certain concentrations of the test object. Rod-shaped bacteria (Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, Serracia marcescens, Escherichia coli, Morganella morganii, Hafnia alvei, Bacillus cereus), coccus microflora (Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecium), different strains of fungi of the genus Candida (Candida non-albicans № 136, 138, 142, 144). The result was evaluated according to the following criteria: “-“ - growth of the test culture (there is no antibacterial effect); “+” - no growth (the antibacterial effect is pronounced); “±” - inhibition of growth (bacteriostatic action).