Определение глутатиона и его окислительно-восстановительного статуса в изолированных вакуолях красных свекольных клеток

Определение глутатиона и его окислительно-восстановительного статуса в изолированных вакуолях красных свекольных клеток

Автор: Прадедова Е.В., Нимаева О.Д., Путилина Т.Е., Семенова Н.В., Собенин А.М., Саляев Р.К.

Журнал: Журнал стресс-физиологии и биохимии @jspb

Статья в выпуске: 1 т.12, 2016 года.

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Глутатион вакуолей красной свеклы (Beta vulgaris L.) измеряли с использованием трех хорошо известных методов: спектрофлуориметрический метод с ортофталевым альдегидом (OPT); спектрофотометрический метод с 5,5'-дитиобис-2-нитробензойной кислотой (DTNB); высокоэффективная жидкостная хроматография (ВЭЖХ). Содержание восстановленного (GSH) и окисленного глутатиона (GSSG) различалось в зависимости от метода исследования. При ОПТ концентрация глутатиона составляла: GSH - 0,05 мкмоль / мг белка; GSSG - 0,019 мкмоль / мг белка и общего глутатиона (GSHtotal) - 0,097 мкмоль / мг белка. В случае определения с DTNB концентрация глутатиона составляла: GSH - 0,091 мкмоль / мг белка; GSSG - 0,031 мкмоль / мг белка; GSHtotal - 0,153 мкмоль / мг белка. Концентрация глутатиона, определяемая по ВЭЖХ, была ниже: GSH - 0,039 мкмоль / мг белка; GSSG - 0,007 мкмоль / мг белка; GSHtotal - 0,053 мкмоль / мг белка. Коэффициент редокс GSH / GSSG также зависит от метода определения: с ОПТ - 3.11; с DTNB - 2,96 и ВЭЖХ - 5,57. Редокс-отношение глутатиона в вакуолях было намного ниже, чем тканевые экстракты красной свеклы, которые в зависимости от метода определения были: 7.23, 7.16 и 9.22. Результаты показали, что вакуоли клеток паренхимы красной свеклы содержат глутатион. Несмотря на низкое значение окислительно-восстановительного отношения GSH / GSSG, в вакуолях пул уменьшенного глутатиона преобладал над пулом окисленного глутатиона.

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Короткий адрес: https://sciup.org/14323988

IDR: 14323988

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