Model constants determination in the DNA melting temperature calculating

The factors affecting the value of the DNA melting temperature T m are described and the degree of their influence is estimated in the article. A review of existing models for calculating the DNA melting temperature is carried out, a significant discrepancy between the results of model application and experimental data is revealed. It’s proposed to use simplified models that take into account the dependence of the temperature T m on some factors with other factors unchanged. A simplified model is given as an example, taking into account the dependence on the length of the DNA fragment and the content of GC-pairs of nucleotides. A method for determining model constants for calculating the DNA melting temperature based on the experimental results analysis and data from the known literature is described. The error in calculating the melting temperature T m when using the model adjusted according to experimental data does not exceed 0.29 °C; the standard deviation is 0.13 °C.