Optimal mode to thaw cryopreserved sperm of Holstein bull sires

As milk yielding increases, the number of animals with defective sexual cycles and quiet manifestations of hunting grows, and therefore there is a need for semen with high quality. Also, at artificial insemination, after dilution of the native semen, packaging, cryopreservation and thawing, the number of spermatozoa per sperm dose decreases. This also necessitates semen with high quality parameters. One of the key stages of the standard processing of cryopreserved bovine semen prior to artificial insemination is the thawing procedure. Therefore, cryopreservation and thawing procedures should be optimized with regard to the proposed modifications of these methods and the changing reproductive abilities of animals. In our study, we compared the effects of previously recommended thawing protocols and those proposed by us on the safety of spermatozoa of Russian Holstein sires and identified the modes that reliably provide a prolonged positive effect on the quality characteristics of spermatozoa and better preservation of their viability after thawing...