Optimization of sigmoid function parameters in real-time pcr signals modeling

Advanced model of real-time PCR signals and methods of its realization on the sigmoid function base is discussed. Numerical experiment showed that the influence of the zero line drift on modeling results can be removed by choosing an appropriate level of threshold model. The criteria of model approximation to actual fluorescence signals growing during the reaction are proposed. Possibility of fully automatic computation of real-time PCR signals threshold cycles was shown.