Optimization of culture media for amidase containing bacteria

The growth characteristics of Gram-negative amidase containing bacteria of Acinetobacter guillouiae llh, Pseudomonas monteilii 5, Alcaligenes faecalis 2 under cultivation in the medium with different concentrations of glucose, sucrose, acetamide and sodium acetate as a carbon were studied. The aim of the work was to optimize the synthetic mineral medium by the source of carbon. It has been determined that the best growth substrate for the manifestation of the amidase activity of the strains was acetamide. With a combination of characteristics such as the biomass yield, the economic coefficient of substrate consumption and enzymatic activity, the optimal acetamide concentration for A. guillouiae llh was 0.025 M, for A faecalis 2 - 0.l M. The growth dynamics of A. faecalis 2 on acetamide in different concentrations was studied. It was shown that the 0.025 M acetamide was insufficient for intensive growth of bacteria, and 0.5 M was inhibitory.