Optimization procedure of metal-affinity chromatography on sorbents, containing ions of ti (iv) for isolation of phosphonylated peptides

The present article describes the optimization procedure of metal-affinity chromatography on sorbents, containing ions of Ti (IV) (Supel-Tips Ti) for separation of peptides from the digest of human serum albumin, incubated with sarin. It is shown that changing the conditions recommended by the manufacturer, namely sorption of alkaline conditions rather instead of acidic, and elution of 0.1 % TFA in 60 % acetonitrile instead of 0.5 % piperidine results in a more efficient isolation of phosphonylation peptides from the samples.