The features of apoptosis activation in seminiferous tubule cells of rats with azoospermia after exposure of beta-radiation at doses of 2 and 8 gray

The development of infertility in male rats under the influence of high doses of ionized radiation is caused not only by direct damage to cells, but also by the block of differentiation and the induction of apoptosis of maturing spermatogonia. However, the literature data on the activity of biological mediators of the internal and external pathways of apoptosis after irradiation with beta particles, in particular, with different effective doses, are extremely limited. Objective: to study the molecular biological mechanisms of apoptosis in the seminiferous tubules of male rats after irradiation with electrons at a dose of 2 and 8 Gray. Male Wistar rats (220±20 g; 9-10 weeks; n=40) were divided into 3 groups: Ist - control (n=10); IInd - electron irradiation, dose 2 Gray (n=15); IIIrd -electron irradiation, dose 8 Gray (n=15). The expression of mRNA of caspases CASP8 and CASP9 was determined on sections of testes with a thickness of 8 pm using real-time polymerase chain reaction. Apoptotic DNA fragmentation was detected by the TUNEL method with the calculation of TUNEL-positive nuclei. On the 50th day after the start of the experiment, a significant number of apoptotic cells were found in the groups of rats subjected to ionizing radiation. The relative number of TUNEL-positive cells of spermatogenic epithelium in the 8 Gray group was 56±1,2% compared with the 2 Gray group - 37±0,9% (p function show_eabstract() { $('#eabstract1').hide(); $('#eabstract2').show(); $('#eabstract_expand').hide(); }