Osteogenesis features for leg lengthening using automatic distractors with the rate by 3 mm for 180 times (an experimental study)

Bone tissue regeneration was studied experimentally during canine leg lengthening by 3 mm in the conditions of day-and-night automatic distraction of high division. By the end of distraction (10 days) a regenerate bone was of sand-glass shape. A high (up to 10-15 mm) connective-tissue layer was represented by fibrils, a great number of the capillaries formed and thin collagen fibers of faint PAS-positive staining, and there were bands of little-differentiated fibroblast-like cells between them which possessed osteogenic potency. All these factors contributed to rapid substitution of the bone tissue for the interlayer after distraction completion, and to formation of an endosteal regenerate bone and an uninterrupted cortical plate after 30 days of fixation. The total period of osteosynthesis in these experiments was 45 days. The results obtained allow to recommend this method for clinical use with the purpose to reduce the periods of treatment in patients subjected to limb lengthening.