Peculiarities of microcirculatory reactions in the area of experimental wound defect in white rats

Objective: to reveal changes of microbloodflow parameters by the laser Doppler flowmetry method in the process of wound defect healing and possibility of their application to modernization of the technologies forwound healing agents effectiveness evaluating. Material and methods. The studies were carried out on 25 white rats divided into 2 groups: 10 control intact rats, 15 animals with a full-thickness experimental skin defect. The microcirculatory parameters of the wound skin edges in rats were evaluated with laser Doppler flowmetry. Histological assay of the wound defect tissues were also performed. Results. It was established that changes in skin microcirculation at the wound defect edges were characterized by inflammatory hyperemia, which was manifested by 27% perfusion increase and increase of normalized amplitudes of myogenic, respiratory and cardiac oscillations. Changes in microcirculation are verified by the morphological picture of inflammation, which reflects an increase in the number of full-blooded vessels of the arterial and venous bed, as well as leukocyte infiltration of the bottom and edges of the wound. Conclusion. Monitoring of microcirculatory disorders occurring in the area of skin wounds allows us to assess the dynamics of the reparative process, which can be used to develop and evaluate the effectiveness of existing drug and non-drug methods of stimulating regeneration.