Some features in preserving actinobacteria of the genus rhodococcus

Viability level necessary to recover cell populations upon long-term storage was measured. It is recommended to preserve alkanotrophic rhodococci pre-cultivated on nutrient hydrocarbon-containing media. The duration of rhodococci storage could be increased using protectants. The most effective lyoprotectants are shown to be a sucrose-gelatine agar or gelatine agar supplemented with Rhodococcus-biosurfactants.