Features of reparative histogenesis in the eardrum remission under the chronic suppurative otitis media

Using the methods of complex (review histologic, histochemical, immunocytochemical and morphometric methods) in an experimental model of chronic suppurative otitis media identified the main factors and regularities in the reparative histogenesis of dogs tympanic membrane tissues. It has been shown that mitotic index in epithelial tissue of the tympanic membrane was greatest at the end of the first week of remission of chronic suppurative otitis media, and then decreased. At the end of the first week it was observed and marked expression of the marker protein content (Ki67). The main proliferation of fibroblasts, morpho-functional organization that testified to their high functional synthetic activity was noted during the first two weeks. The results of myringoplasty conducted using nanostructured bioplastic-material based on hyaluronic acid in the various terms of remission of chronic suppurative otitis media showed that the best results are observed at myringoplasty its holding in the first two weeks after the inflammation subsided. The results showed that, to ensure optimal conditions for the implementation of the histogenesis of reparative tissue of the tympanic membrane in a remission of chronic suppurative otitis media must be no pronounced inflammation in the middle ear, the implementation myringoplasty in the first weeks of remission, as well as the use of bioplastic material bidegradiruemogo myringoplasty goals.