Acute inhalation toxicity of new disinfectant

The aim of the study is to investigate the acute inhalation toxicity of the disinfectant coded TN, developed at the Federal Center for Veterinary and Traumatological Research FSBSI FCTRBS-ARRVI for veterinary use in the presence of animals with determination of the toxicity class. The study was conducted in the veterinary sanitation laboratory of the Federal Center for Veterinary and Traumatological Research (Kazan). The object of the study was the disinfectant TN, which is a liquid containing (30.0 ± 5.0) % 1,3,6,8-tetraazatricyclododecane and 0.1 % oxyethylated nonylphenol, a nonionic surfactant. White rats were used in the experiment, of which 2 groups were formed - control and experimental. The study was conducted in an inhalation baiting chamber. A 4.0 % solution of the disinfectant TN was introduced into the chamber with the experimental animals at a rate of 0.1 l of the working solution per 1 m3. The rats of the control group were also placed in the inhalation baiting chamber, but without the supply of the test solution. The exposure in the chamber was 4 hours, and the animals were observed for the next 14 days. This type of exposure to experimental animals did not have a negative impact: no deaths of rats were recorded, the general condition in the experimental and control groups remained satisfactory throughout the observation period, hematological, biochemical and pathomorphological parameters of rats in both groups were within the physiological norm. The hazard class according to GOST 32646-2014 is not classified. These data allow us to consider the TN agent as a promising disinfectant, which can be used in the presence of animals after further comprehensive study of general and specific toxicity.