The influence of nitric oxide (II) donors on some biochemical parameters of blood and urine in a model of chronic renal failure

Studies were conducted on female rats of Wistar weighing 220-250 g, which were formed into 3 groups of 5 rats in each: I - intact, II - control with model chronic renal failure (RF); III -experimental with model chronic RF and with introduction the solution of L-arginine at dose of 200 mg/kg intraperitoneally. Chronic RF was modeled by the introduction of a 50% aqueous solution of glycerol intramuscularly (10 ml/kg mass of the hind limbs). Blood was taken from the caudal vein. Urine sampling was performed after 2 months in the special cages. The concentration of indicators in serum and urine was determined by colorimetric methods on biochemical analyzer "Bi-An" (Russia) with the set of reagents ("Olvex", Russia). It was found that the introduction of nitric oxide (II) donors restores the excretion of urea, creatinine and chlorine ions and can be used as a treatment supporting kidney function in chronic renal failure.