Nutritional and protective environmental factors for microbial consortium cultivation

Currently, the use of highly active probiotic strains for obtaining consortia with high biochemical and technological potential is relevant and promising in the dairy industry. Such probiotic microorganisms include propionic acid bacteria and Acidophilus bacillus. The creation of a consortium based on Propionibacterium freudenreichii Sh 85 and L. acidophilus makes it possible to develop specialized food products and biologically active additives with high probiotic properties. An inoculate was obtained based on pure cultures of Propionibacterium freudenreichii Sh 85 and L. acidophilus in a ratio of 2:1, which has a high biochemical activity and can provide a high level of enzymatic processes when co-cultured on a dairy medium. The nutrient medium based on whey has been optimized for obtaining the biomass of a consortium of propionic acid bacteria and Acidophilus bacillus. The optimal temperature range for biomass growth is (30±2) °C. The article shows the results of the selection of the optimal amount of cobalt ions in the nutrient medium. For normal biomass growth, high yield of viable cells of Propionibacterium freudenreichii Sh 85 and L. acidophilus cultures and vitamin B12 biosynthesis, a dose of 0.0025 g/l cobalt chloride was selected. The resulting biomass is characterized by a high titer of viable cells of propionic acid bacteria and Acidophilus bacillus 1012 k.f.u./cm3. The accumulation of vitamin B12 is active in the biomass. Cellular biomass is applicable as a biologically active additive for direct consumption and in the dairy industry as a bacterial concentrate.