Optimal parameters selection for extraction of biologically active compounds complex from medicinal plants suspension cultures of the Siberian federal district

The study was carried out to optimize the extraction of plant raw materials, presented in the form of dried biomass of suspension cultures of cells of medicinal plants of the Siberian Federal District: Scutellariagalericulata, ScutellariaBaicalensis, Potentillaalba, Schisandra, Platantherabifolia and Codonopsispilosula. The variable parameters of the extraction process were the duration of the extraction, the temperature regime, and the ratio of the volume of organic solvent to the amount of dried biomass of suspension cultures of plant cells. A more effective organic solvent for obtaining extracts of ScutellariaBaicalensis and Scutellariagalericulata is 70 % ethanol, Platantherabifolia and Schisandra – acetone, Potentillaalba – diethyl ether, Codonopsispilosula – ethyl acetate. During the experiment, the maximum yield of dry extract was estimated. The optimal parameters for the extraction of the complex of biologically active substances from the dried biomass of the suspension cultures of the medicinal plant ScutellariaBaicalensis are the temperature 50 °C, duration 60 minutes, hydromodule 1:10, Potentilla alba – temperature 40 °C, duration 30 minutes, the ratio of the solvent volume to the initial mass raw materials 1:20, Scutellariagalericulata – temperature 60 °С, duration 30 minutes, hydromodule 1:10, Schisandra–hydromodule 1:5, duration 30 minutes and the process temperature is 40 °C, any Platantherabifolia– the ratio of the volume of the solvent to the mass of the culture is 1:5, the duration of the extraction is 30 minutesand a temperature of 40 °C, Codonopsispilosula –the process temperature is 40 °C, the duration of the extraction is 30 minutes, the hydromodule is 1:10. Optimization of the extraction process of bio-logically active substances allows increasing the economic component when using technologies in industrial production.