Selection of a system for apparatus cultivation of suspension cultures of cells and root cultures in vitro in bioreactors of various designs and volumes
Автор: Asyakina L.K., Dyshlyuk L.S., Pozdnyakova A.V., Prosekov A.Y.
Рубрика: Биохимический и пищевой инжиниринг
Статья в выпуске: 3 т.9, 2021 года.
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The production of biologically active substances from medicinal plants is currently an urgent task. The ways of obtaining these substances are varied. This article discusses the instrumental cultivation of suspension cultures of cells and root cultures in vitro in bioreactors of scutellaria baicalensis and scutellaria andrachnoides. For cultivation the following are offered: liquid-phase column-type bioreactor with mechanical stirring (stirring speed 50 rpm), working volume 30 l; liquid-phase column-type bioreactor with bubbling (bubbling of sterile air or gas mixture supplied from below), working volume 50 l; drum-type liquid-phase bioreactor, working volume 100 l; fog-type gas-phase bioreactor (diameter of nutrient medium droplets 20-25 microns), working volume 30 l; sprinkler-type gas-phase bioreactor (diameter of nutrient medium droplets 500 microns), working volume 30 liters. Data were obtained that the cultivation of suspension cultures of scutellaria andrachnoides and scutellaria baicalensis is the use of a liquid-phase column-type bioreactor with a bubble gum, since in this case the maximum values of the growth index, specific growth rate and economic coefficient, as well as the total content of the main biochemical compounds. In the root culture of scutellaria baicalensis, grown in gas-phase bioreactors of the fog and sprinkler type, the maximum content of the main biochemical compounds was recorded. For the root culture of scutellaria andrachnoides, the highest content of biochemical compounds was noted during cultivation in a fog-type gas-phase bioreactor.
Scutellaria baicalensis, scutellaria andrachnoides, bioreactor, suspension cell cultures, in vitro root cultures, media composition, growth characteristics
Короткий адрес: https://sciup.org/147235230
IDR: 147235230 | DOI: 10.14529/food210305