Selection of the ratio of microbial components to accelerate the biodegradation of bird droppings

The aim of presented studies was to select the optimal ratio of the proteolytic culture of Pseudomonas putida 90 biovar A (171) and a strain that has the properties of fixing atmospheric nitrogen - Azotobacter chroococcum 31/8 R to accelerate the process of biodegradation of chicken droppings to indicators of the relevant regulatory documentation. In conducted studies, the following indicators were analyzed: content of ammonium nitrogen, total microbial number, ammonia nitrogen, index of bacteria of the escherichia coli group, index of enterococci and pathogenic microorganisms, eggs and larvae of helminths, and the hazard class of processed and untreated chicken droppings was determined by the calculation method. As a result of conducted physico-chemical and sanitary-biological analyses, it was found that a more optimal and stable result was revealed when processing chicken droppings with the studied cultures in a ratio of 1:1. This treatment of droppings with cultures contributed to a 15-day decrease in the level of ammonium nitrogen in the by-product from 278 mg/l to 97 mg/l, the content of ammonia in the environment from 84 mg/m3 to 13 mg/m3, index of bacteria of the escherichia coli group from 4 to 1 units, index of enterococci from 5 to 2 units, index of pathogenic microorganisms from 2 to 0 units, number of eggs and larvae of helminths from 8 to 0 copies, number of larvae of synanthropic flies from 2 to 0 copies at the same time increasing the total microbial number to a value of at least 1011 cells/g. The hazard class of chicken droppings treated with microbial research objects decreased from III to IV and met the requirements of GOST 31461-2012.